Radioiodination of proteins by the use of the chloramine-T method. Amber mutation affecting the length of Escherichia coli cells. Organization of genes in the ftsA-envA region of the Escherichia coli genetic map and identification of a new fts locus (ftsZ). Lutkenhaus JF, Wolf-Watz H, Donachie WD.Coupling of DNA replication and cell division: sulB is an allele of ftsZ. Maturation of the head of bacteriophage T4. Identification of the Escherichia coli cell division gene sep and organization of the cell division-cell envelope genes in the sep-mur-ftsA-envA cluster as determined with specialized transducing lambda bacteriophages. Fletcher G, Irwin CA, Henson JM, Fillingim C, Malone MM, Walker JR.Cell length, cell growth and cell division. Role of the ftsA gene product in control of Escherichia coli cell division. Donachie WD, Begg KJ, Lutkenhaus JF, Salmond GP, Martinez-Salas E, Vincente M.Production of thiol-penicillin-binding protein 3 of Escherichia coli using a two primer method of site-directed mutagenesis. Interaction of nocardicin A with the penicillin-binding proteins of Escherichia coli in intact cells and in purified cell envelopes. Identification of new genes in a cell envelope-cell division gene cluster of Escherichia coli: cell division gene ftsQ. Cell shape and division in Escherichia coli: experiments with shape and division mutants. Linkage map of Escherichia coli K-12, edition 7. Links to PubMed are also available for Selected References. Get a printable copy (PDF file) of the complete article (1.9M), or click on a page image below to browse page by page.
PBP3 MUTATION E. COLI FULL
Full textįull text is available as a scanned copy of the original print version. These observations suggested that the FtsA protein, a protein with a structural and regulatory role in septation, and PBP3, a protein enzymatically active in the synthesis of murein for septation, interact with each other. A molecular species able to cross-react with an anti-PBP3 serum was nevertheless found to be present in the envelope of D-3 cells. The binding of a radioactively labeled derivative of ampicillin to penicillin-binding protein 3 (PBP3) was significantly decreased in strain D-3, containing the mutant allele ftsA3, when the binding assay was performed at the restrictive temperature. Potential septation sites in the filaments of wild-type cells treated in the same way remained inactive. The ftsA2 allele codes for an FtsA protein which is inactive at 42 degrees C but is able to regain its activity once it is transferred back to 30 degrees C ftsA2 filaments formed at 42 degrees C in the presence of penicillin divided once the penicillin was removed and the temperature was lowered to 30 degrees C. Mutations in the ftsA gene of Escherichia coli conferred a higher resistance to lysis induced by penicillin or by a combination of cefsulodin and furazlocillin.
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